Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples.
نویسندگان
چکیده
A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(-1) in artificially inoculated bovine feces following enrichment.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 70 3 شماره
صفحات -
تاریخ انتشار 2004